Isolation and Structural Characterization of a Steroidal Antimicrobial Agent from
نویسندگان
چکیده
The preliminary phytochemical screening of the root of Clerodendrum baronianum and antimicrobial activities of the crude extract in various solvent systems were determined. A pure colorless needle shape crystal compound (CB) was separated by thin layer and column chromatographic methods. The yield is found to be (10.3 mg %) based upon the ethyl acetate crude extract. The melting point of this compound is 251-253°C. The crude extract and pure compound (CB) were screened for the antimicrobial activity employing several microorganisms. This compound (CB) showed the highest antibacterial activity against B. subtilis, E. coli, P. aeruginosa and St. aureus. The phytochemical analysis showed that the active agent is a steroid compound. Furthermore, the molecular formula of this pure compound might be assigned as C H O N based upon the 28 46 2 results of several advanced spectroscopic analysis such as IR, HNMR, CNMR and EI-MS. 1 13
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